Peptide and Protein Quantification Using iTRAQ with Electron Transfer Dissociation
نویسندگان
چکیده
منابع مشابه
Peptide and protein quantification using iTRAQ with electron transfer dissociation.
Electron transfer dissociation (ETD) has become increasingly used in proteomic analyses due to its complementarity to collision-activated dissociation (CAD) and its ability to sequence peptides with post-translation modifications (PTMs). It was previously unknown, however, whether ETD would be compatible with a commonly employed quantification technique, isobaric tags for relative and absolute ...
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This tutorial review describes the principles and practices of electron capture and transfer dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide and protein structure analysis. ExD MS relies on interactions between gas phase peptide or protein ions carrying multiple positive charges with either free low-energy (~1 eV) electrons (ECD), or with reagent radical anions possess...
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ژورنال
عنوان ژورنال: Journal of the American Society for Mass Spectrometry
سال: 2008
ISSN: 1044-0305
DOI: 10.1016/j.jasms.2008.05.023